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Water documentary - building a bandpass filter for viewing fluorescein in plants?


Catherine1

Hi, 

I hope you can help me. I work for a film production company in London called Passion Planet and we are making a documentary series about water. We have a crew travelling to the Amazon rainforest, leaving this Monday 22nd October, and while we’re in the field we are hoping to film an experiment viewing fluorescein dye in a plant. 

I am trying to create a way to illuminate the plant. The excitation peak of the dye is 489 nm, The emission peak is 509 nm.  

So far I have found the following on the RS website: 

1. Blue LED e.g. Cree XPEBLU RS Stock No. (810-7064)   

2. LED driver e.g. Philips 17 W RS Stock No. (722-7902)  

I am hoping to connect the LEDs in series so that they are all powered by the same driver. But I can't see what connectors would be appropriate. Also, it doesn't look like RS stock a driver connector (something like this https://www.ledsupply.com/accessories/xitanium-driver-connector ?) Do I need one? 

Ideally we'd be able to illuminate the plant from several angles. 

Also, I'm looking to place the LEDs in a spot lens to help illumination but is it still possible to attach a heatsink? 

I don't have any store cupboard equipment so need to buy everything - any advice would be really appreciated. 

Thank you in anticipation, 

Catherine Watling 

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